Figure 3

IEGs are transcriptionally induced by CPT treatment. DIV30 neurons were treated and subjected to exon array analysis as in Fig. 2. (a) Heat map depicting the expression of IEGs induced by CPT, in relation to ATM activity. (b) qRT-PCR analysis of ARC, EGR1, FOS and GAPDH (the latter used for normalization) performed on mRNA extracted from WT1 and A-T1 neuronal preparations. Data are average of six experiments +/−SD and results normalized against those of GAPDH (* p < 0.05; **p < 0.01).