Figure 6
Physiological stimulation of neuronal activity induces a DDR signalling. DIV55 neurons were treated with NMDA or KCl for up to 30 min and analysed for the phosphorylation of ATM targets. To inhibit action potential, neurons were pre-incubated overnight with the sodium channel blocker TTX. (a) Time-dependent phosphorylation of ATM targets induced by NMDA. Pre-incubation for 1 hr with the NMDA receptor antagonist MK801 was also performed (lane 5). (b) The ATMi was added 1 hr prior to treatment for 20 min with NMDA or KCl. (c) Time-dependent phosphorylation of ATM targets upon KCl-depolarization. Vinculin and β-Actin are loading controls for proteins.
