Figure 7

Neuronal activity enhances the expression of ARC and c-Fos proteins DIV55 neurons were incubated overnight with TTX, thereafter with KCl for up to 4hrs and assessed by Western blot for c-Fos and ARC proteins. Note the ATM-independent induction of these IEGs, as also evident from the WT1 treated with ATMi (right panel). Vinculin and β-Actin are loading controls for proteins.