Figure 5
SNAIL is required for TGF-β1-induced loss of Müller glial property. (A,B) Müller glial cells were treated with or without 10 ng/ml TGF-β1. (A) Real-time quantitative PCR analyses for the time course of expression levels of GLUL. n = 6 per group, *p < 0.05, **p < 0.01. (B) Immunoblot analyses for the time course of protein expression of GS and GAPDH. h, hours. Full size blots are shown in Supplementary Fig. S3A. (C–E) Control siRNA-treated (Ctrl-siRNA) and SNAI1-knockdown (siRNA-1 and -2) Müller glial cells were treated with or without 10 ng/ml TGF-β1 for 24 hours. (C) Real-time quantitative PCR analyses for GLUL. n = 6 per group, *p < 0.05. (D) Immunoblot analyses for GS and GAPDH. Full size blots are shown in Supplementary Fig. S3B. (E) Immunocytochemical analyses for GS. Scale bar = 50 μm.
