Figure 1
Cytokine production by THP-1-derived macrophages and monocyte-derived dendritic cells in response to E. gallinarum MRx0518. IL-8, TNFα, IL-6, IL-10, IL-12p70, IL-23, IL-1β concentrations (pg/ml) in (A) THP-1-derived macrophages and (B) monocyte-derived DCs cell-free supernatants after 1 h contact with E. gallinarum MRx0518 (MRx0518LV). A multiplicity of infection (MOI) of 10:1 was employed for both stimulation assays. A MOI of 1:1 was employed for IL-8 detection in macrophages, due to saturation of the assays at the MOI of 10:1. The effect of LPS on cytokines production is also shown as a positive control. The graphs represent an average of at least three biological replicates. Statistical comparisons were performed with GraphPad Prism (La Jolla, CA, USA) using ordinary one-way ANOVA analysis followed by Tukey’s multiple comparison tests. Statistically significant differences with the untreated control are shown on the graphs as *(p < 0.05), **(p < 0.01), ***(p < 0.001) and ****(p < 0.0001).
