Figure 4
Flagellin plays a role in E. gallinarum MRx0518 immunostimulatory effect. NF-κB (A) and TLR5 (B) reporter assays with MRx0518 (MRx0518SN), MRx0518 fliC::pORI19 (fliCSN) and DSM100110 (DSM100110SN) culture supernatants (MOI 100:1 equivalent). NF-κB (C) and TLR5 (D) reporter assays with a range of concentrations of E. gallinarum MRx0518 and DSM100110 purified recombinant flagellins (FliCMRx0518 and FliCDSM100110). The ‘Control’ bar corresponds to the empty vector control. Each graph represents an average of at least three biological replicates. Reporter cells were incubated with treatments for 22 h. Statistical comparisons were performed with GraphPad Prism using ordinary one-way ANOVA analysis followed by Tukey’s (A,B) or Sidak’s (C,D) multiple comparison tests. Statistically significant differences with the relevant control are shown on the graphs as *(p < 0.05), ***(p < 0.001) and ****(p < 0.0001).
