Figure 4 | Scientific Reports

Figure 4

From: Increased throughput and ultra-high mass resolution in DESI FT-ICR MS imaging through new-generation external data acquisition system and advanced data processing approaches

Figure 4

Mass spectral images of lipid species detected around m/z 828.55 in ~1 scan per second experiments on adjacent rat kidney sections. Top-right shows the spectrum around m/z 828.55, for the conventional LTQ-FT setup (blue) and the external DAQ (red). The achieved mass resolution on the most abundant m/z 828.55 peak was 24.4 k and 156 k, respectively (average spectrum of entire sample area). Image recording took 2 hours for each image at 150 µm by 150 µm pixel size, with both datasets containing approximately 8000 pixels. All images are TIC normalized and plotted on a linear intensity scale. Given the very low abundance of some species, a 5% image intensity threshold was applied to each image to reduce noise. The full profile aFT version of (b) is provided as Supplementary Fig. S2.

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