Figure 3
From: Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses
LPS-induced responses of various ex vivo primary cell cultures derived from the hTNF.LucBAC mouse strain. Luciferase activity as detected in peritoneal macrophages (a) or splenocytes (b) without stimulation (dotted line) or after stimulation (solid line) with LPS (10 ng/mL); data are representative of three independent experiments, with triplicate cultures per experiment (N = 3, n = 3). Tnf and luciferase mRNA abundance as detected in LPS-stimulated peritoneal macrophages (c) and splenocytes (d) over 6 h (N = 3 mice). Soluble TNF released to the culture medium in response to LPS treatment of peritoneal macrophages (e) and splenocytes (f) over 24 h (N = 6 mice). Bars represent standard error of the mean. Significant differences from unstimulated controls, *p < 0.05, **p < 0.01, ***p < 0.001; ANOVA.
