Table 1 Primer sequences and the labeled fluorophores.

From: A novel emulsion PCR method coupled with fluorescence spectrophotometry for simultaneous qualitative, quantitative and high-throughput detection of multiple DNA targets

Primer name

Sequences

Fluorophores

Excitation Wavelength

Emission Wavelength

Bt176-F

5′-AAGCACGGTCAACTTCCGTAC-3′

5′ FAM

470 nm

522 nm

Bt176-R

5′-TCGACTTTATAGGAAGGGAGAGG-3′

   

GA21-F

5′-CTTATCGTTATGCTATTTGCAACTTTAGA-3′

5′HEX

505 nm

556 nm

GA21-R

5′-TGGCTCGCGATCCTCCT-3′

   

NK603-F

5′-CGGTACCAAGCTTTTATAATAGTAG-3′

5′ ROX

550 nm

602 nm

NK603-R

5′-CTAGTCTGTTATGGTTCGAG-3′

   

TC1507-F

5′-GCCAGTTAGGCCAGTTACCCA-3′

5′ NED

530 nm

575 nm

TC1507-R

5′-CAAGATCAAGCGGAGTGAGG-3′

   

IVR-F

5′-GTATCACAAGGGCTGGTACC-3′

5′ CY5

630 nm

665 nm

IVR-R

5′-CCGTGTAGAGCATGACGATC-3′

   

Salmonella-F

5′-TTGTGCCGAAGAGCCGGCGT-3′

5′ FAM

470 nm

522 nm

Salmonella-R

5′-TTGCGAATAACATCCTCAAC-3′

   

L. monocytogenes-F

5′-GGTTTAGCTTGGGAATGGTG-3′

5′HEX

505 nm

556 nm

L. monocytogenes-R

5′-GATAAAGCGTAGTGCCCCAG-3′

   

E. coli-F

5′-CGTCGTGTCTGCTAAAAC-3′

5′ ROX

550 nm

602 nm

E. coli-R

5′-GGTTGCTTGCGTTTGAGAC-3′

   

S. aureus-F

5′-AAATCCAGCACAACAGGAAACGACACA-3′

5′ NED

530 nm

575 nm

S. aureus-R

5′-ATCTCCGGCCATAATTGGTGGCACT-3′

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