Figure 3
Quantitative analysis of the morphological parameters of primary dendritic spines between control and ASD neurons. (a) Primary spine segments with corresponding 3D reconstructions. Scale bar = 2 μm. Dendrite segments (grey color) are endowed with four categories of spines: Filopodia (pink color), Thin (blue), Stubby (pink) and Muschroom (green). (b) Spine morphological parameters were quantified using the Imaris sofware as described in Materials and Methods. Numbers of neuronal dendrites are indicated in the graph. Data are presented as mean ± SEM. Statistical analysis was performed using unpaired Student t-test in order to analyze the significance between mean values from combined controls and combined patients. Equality of variances was checked using the Fisher’s F-test. P values are directly indicated in the graph. *p < 0.05, **p < 0.01.
