Figure 4

Morphological classification of primary dendritic spines between control and ASD neurons. Morphological parameters of the four spine categories were quantified using the Imaris sofware as described in Materials and Methods. Data are presented as mean ± SEM. Statistical analysis was performed using unpaired Student t-test. Equality of variances was checked using the Fisher’s F-test. P values are directly indicated in the graph. Combined mean values for Filopodia were significantly increased as compared to combined values from all other spine categories (p < 0.0001). Statistical analysis was performed as described above.