Table 2 Enzymes and substrates.

Enzyme	Substrate	Ex/Em [nm]	Turnover
*Serine proteinases*
trypsin^a	Boc-FSR-Amc^b	380/460	trypsin^a
plasmin^c
t-PA^d
chymotrypsin^e	AAF-Amc^b	380/460	chymotrypsin
*Cysteine proteinases*
papain^f	Boc-FSR-Amc^b	380/460	trypsin^a
cathepsin B^g
cathepsin K^g
cathepsin S^g
legumain^h	Z-AAN-Amc^b	380/460	legumain
*Aspartate proteinases*
cathepsin D^g	Mca-GKPILFFRLK(Dnp)-dR-NH₂^g	328/405	proteinase K^f
*Metalloproteinases*
MMP-2^g	Mca-PLA-Nva-Dap(Dnp)-AR-NH₂^h	320/405	proteinase K^f
MMP-8^h
MMP-9
MMP-13^h	Mca-PLGL-Dap(Dnp)-AR-NH₂^h	320/405	proteinase K^f
ADAM10^h	Mca-PLAQAV-Dpa(Dnp)-RSSSR-NH₂^h	320/405	proteinase K^f
TLL-2	Ac-RE(Edans)-DR-Nle-VGDDPY-K(Dabcyl)-NH₂ⁱ	350/520	proteinase K^f
BMP-1
ovastacin
meprin α
meprin β
astacin	Dns-PKRAPWVⁱ	280/350	proteinase K^f
astacin	Mca-GSPAFLA-K(Dnp)-dR-NH₂^g	320/405	proteinase K^f
nephrosin	Mca-PLAQAV-Dpa(Dnp)-RSSSR-NH₂^h	320/405	proteinase K^f

^aMerck, Darmstadt, Germany; ^bBachem, Bubendorf, Switzerland; ^cABCAM Cambridge, UK; ^dHematologic Technologies, Vermont, USA; ^eServa, Heidelberg, Germany; ^fSigma-Aldrich, Taufkirchen, Germany; ^gEnzo Life Sciences, Lörrach, Germany; ^hR&D Systems Europe Ltd, Oxon, UK; ⁱBiosyntan, Berlin, Germany; Ac: acetyl; ADAM: A Disintegrin and Metalloproteinase; Amc: 7-amido-4-methylcoumarin; BMP: bone morphogenetic protein; Boc: t-butyl-oxycarbonyl; Ex: excitation wave length; Em: emission wave length; Mca: 7-methoxycoumarin-4-yl-acetyl; Dnp: 2,4-dinitrophenyl; Dap: L-2,3-diaminopropionyl; Dap(Dnp): L-2,3-diaminopropionyl-N3-2,4-dinitrophenyl; EDANS: 5-((2-aminomethyl)amino)naphtalene-1-sulfonic acid; Dans: N,N’-dimethylamino-naphtalene-1-sulfonic acid; MMP: matrix metalloproteinase; Na: nitroanilide; Nva: norvaline; Dabcyl: N,N’-diamino-phenyl-azo-4-benzoic-acid; Suc: succinyl; t-PA: tissue-type plasminogen activator; Z: benzyloxycarbonyl. ‘Turnover’ indicates the enzyme used (at 20 μg/1 μl) for complete substrate turnover after initial rate determination.

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