Table 2 Permutation multivariate analysis of distance matrix (Bray-Curtis).

From: Microbial embryonal colonization during pipefish male pregnancy

 

Model

Dev. stage

Bact. treat

Dev. stage x Bac. treat

Matrix

Residuals

F.Model

R2

P(>F)

F.Model

R2

P(>F)

F.Model

R2

P(>F)

BrayCurtis

0.655

6.26

0.24

<0.001***

2.19

0.02

0.207

2.03

0.08

0.001**

Residual Degrees of Freedom

DF = 67

DF = 4

DF = 1

DF = 4

Total Degrees of Freedom

DF = 76

  1. Multivariate PERMANOVA analysis to assess the effect and interaction of the two fixed factors developmental stage (Dev. stage) x parental bacteria treatment (Bac. treat) while implementing tank as strata term with p-values obtained by 10000 permutations. Significant p-values are marked in bold letters (significance code: <0.001***, 0.001**, 0.01*). Each analysis was based on OTU distance matrices obtained by tools implemented in MOTHUR (1419 OTUs, subsamples > 6000).