Figure 2 | Scientific Reports

Figure 2

From: Suppression of SIRT1 in Diabetic Conditions Induces Osteogenic Differentiation of Human Vascular Smooth Muscle Cells via RUNX2 Signalling

Figure 2

Hyperglycaemic conditions induce smooth muscle cell mineralisation. The effect of control (Ctrl), CaCl2 and BGP osteogenic (Ost) and osteogenic with high glucose (HG) conditions was assessed. (a) Representative micrographs (n = 4 and 5FoV) show positive Alizarin red staining in osteogenic conditions by day 21, which is significantly greater in high glucose osteogenic media. (b) ALP activity was increased by day 4 in hyperglycaemic conditions and increased by over a two-fold in hyperglycaemic conditions compared to osteogenic conditions by day 7 (n = 5). (c) MSX2 mRNA expression significantly increased in hyperglycaemic conditions compared to control (n = 3). *P < 0.05, **P < 0.005, ***P < 0.001. Scale Bars = 100 μm

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