Table 3 Summary of CRISPR-Cas9 genome mutagenesis in A. rhodensis, A. freiburgensis and S. stercoralis.
Target gene | Life stage injected | Lip (+/−) | P0 rolling (%) | F1 rolling (%) | F2 rolling (%) from rolling F1 parent | |
|---|---|---|---|---|---|---|
P0 rolling | P0 wild-type | |||||
Arh-rol-6.1 | Adult (H) | Lip+ | 77 (n = 27) | 100 (n = 1144) | 0 (n = 466) | 100 (n = 2539)* |
Adult (H) | Lip− | 0 (n = 13) | NA | 0 (n = 1173) | NA | |
Larval (L2d) | Lip+ | 80 (n = 20) | 100 (n =  1658) | 0 (n = 885) | ND | |
Larval (L2d) | Lip− | 0 (n = 8) | NA | 100 (n =  1958) | ND | |
Afr-rol-6.1 | Adult (H) | Lip+ | 37 (n = 27) | 100 (n = 1802) | 0 (n =  1202) | ND |
Adult (H) | Lip− | 0 (n = 10) | NA | 0 (n > 1000) | ND | |
Sst- SSTP-0000742500 | Adult (F) | Lip+ | 0 (n = 15) | NA | 4 (n = 200) | NA |
Larval (iL3) | Lip+ | 13 (n = 23) | 0 (n = 5) | ND | NA | |
