Figure 4
From: TMEM16F activation by Ca2+ triggers plasma membrane expansion and directs PD-1 trafficking
Membrane expansion occurs in parallel with conductance and current changes and requires the presence of monovalent cations. (A) Single Jurkat T cells were patched with a glass micropipette loaded with cytoplasmic solution (see Materials and Methods) and incubated at 37 °C in Ringer’s solution. Cells were treated with 5 μM ionomycin for the period shown. Measurements were made of total capacitance, Cm, transmembrane conductance, Gm (lower blue trace), and transmembrane current Im (lower green trace). The upper red trace shows the change in capacitance (∆Cm), while the upper blue trace shows the integral ∫Gm. Records were scaled to start and end at the same time, n = 10. (B) Membrane expansion was recorded as in ‘A’ and monovalent cation concentrations employed were varied as indicated.
