Figure 4
From: Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities
Denaturant effect of MBP unfolding and MBP-maltose binding. (A) Tm of MBP decreases with increasing GdnHCl concentration. (B) MBP-maltose binding with 0.7 M GdnHCl at 35 °C. The value of Kd is 2.3 µM. All the experiments were carried out in triplicate. The protein concentration in all the assays was fixed to 2 µM. All assays were taken in the buffer: 120 mM NaCl, 20 mM NaH2PO4/Na2HPO4, pH 7.4 with 1% DMSO and the melting program was set to 0.5 °C/min.
