Table 1 Binding/inhibition constants derived from isothermal analysis of DSF and from enzyme activity assays.

From: Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities

Interaction (ligand/protein)

Kd (µM), from DSF

Ki (µM), from enzyme assay

SULFA/h-CA I

1134 ± 106

786 ± 60

SULFA/b-CA II

106 ± 9

136 ± 5

ACTAZ/h-CA I

7.2 ± 0.8

2.1 ± 0.2

ACTAZ/b-CA II

<0.5

0.48 ± 0.03

METAZ/h-CA I

1.2 ± 0.1

1.9 ± 0.2

METAZ/b-CA II

0.35 ± 0.03

0.52 ± 0.03

TFMSA/h-CA I

<0.5

<1

TFMSA/b-CA II

<0.5

0.12 ± 0.04

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