Figure 1

MSA increases adult DG neuronal differentiation in rats. (a) Schematic diagram outlining the experimental procedures of MSA and neuronal differentiation assay. (b) Rats acquire a stable preference for the morphine-coupled hole. (i) Infusions of SSA and MSA. Treatment × Day: ^*p < 0.05; two-way repeated measures ANOVA. (ii) Inactive nosepokes of SSA and MSA. (c) MSA increases neuronal differentiation in the adult DG. (i) Representative images in each SA group. Confocal illustration shows colocalization of the neuronal marker NeuN (red) and the cell division marker BrdU (green). Scale bar represents 50 μm. (ii) MSA does not alter the number of BrdU⁺ cells in the DG. (iii) MSA increases neuronal differentiation in the DG. Number of brains scored (number of BrdU⁺ cells examined): SSA = 7 brains (279 cells); MSA = 8 brains (338 cells). ^*p < 0.05; unpaired student’s t test. (d) Schematic diagram outlining the experimental procedures of MSA and dendritogenesis assay. (e) Rats acquire a stable preference for the morphine-coupled hole. (i) Infusions of SSA and MSA. Treatment × Day: ^****p < 0.0001; two-way repeated measures ANOVA. (ii) Inactive nosepokes of SSA and MSA. (f) MSA increases dendritic complexity of neurons in DG. (i) Representative images and traced counterparts in each group. Scale bar represents 20 μm. (ii) Sholl analysis of EGFP⁺ cells: Treatment × Distance: *p < 0.05, two-way repeated measures ANOVA. All data are presented as mean ± s.e.m.