Figure 7
Thin layer chromatography and GC-MS study of fungal ME, and its effect in reduction of the phenoloxidase inside the anopheline larvae. (a) Spots of the MF8 of T. asperellum (TaspSKGN2) in TLC plates under UV (254 nm & 366 nm) and within an iodine vapor chamber. (Rf value: 0.83) [i. Spot under UV at 254 nm ii. Spot under UV at 366 nm iii. Spot within Iodine vapour chamber]. (b) GC-MS chromatogram of the MF8 of T. asperellum ME extracts exhibiting peaks of 49 compounds. (c) Graphical representation of the reduction in phenoloxidase activity after the initial increase inside the anopheline larvae (N = 10) treated with the LD50 dose of ME of T. asperellum in different interaction times.
