Figure 3 | Scientific Reports

Figure 3

From: RgsD negatively controls development, toxigenesis, stress response, and virulence in Aspergillus fumigatus

Figure 3

RgsD downregulates a cAMP-PKA signaling pathway. (A) Expression of gpaB, acyA, pkaC1, and pksP mRNA in three strains analyzed by qRT-PCR. Student’s t-test: **p < 0.01. (B) PKA activity of three strains as monitored by gel electrophoresis. A phosphorylated substrate migrates toward the anode (+). Each strain was grown in MMG for indicated time at 37 °C and mycelial extract was analyzed. (C) Kinase activity was determined by the spots (panel B) quantification using spectrophotometer. Note that mRNA levels of cAMP-PKA signaling pathway related genes and PKA activity at 24 hour were significantly increased in the ΔrgsD mutant compared to WT and C′ strains.

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