Figure 4

Effects of 4-week butyrate supplementation on monocyte training capacity. In freshly isolated adherent monocytes from healthy lean volunteers (Lean) and metabolic syndrome patients (MetSyn) training was induced as depicted in Fig. 3. This experiment was performed before and after 4-week supplementation with 4 grams oral butyrate daily. (A) A heatmap shows the mean fold decrease or increase in cytokine production with training after supplementation in both groups. (B–G) Stimuli-cytokine combinations showing a substantial decrease or increase in panel A are highlighted in separate graphs to show the fold change with training at baseline (BL) and 4 weeks (4 w). Lean n = 7, MetSyn n = 7.