Table 1 Codes and details of larval culturing and comparisons performed in molecular analyses, plus the number of annotated transcripts up-regulated in the treated samples when searched against the (A) GenBank non-redundant and (B) Swissprot human databases.

From: Molecular mechanisms underpinning transgenerational plasticity in the green sea urchin Psammechinus miliaris

Time

Treatment

A: GenBank non-redundant

B: Swissprot human

2 days

Ambient adults/low pH larvae

CE2

204

91 (81)

Low pH for both adults and larvae

E2

274

117 (109)

3 days

Ambient adults/low pH larvae

CE3

92

49 (43)

Low pH for both adults and larvae

E3

355

153 (138)

4 days

Ambient adults/low pH larvae

CE4

469

315 (309)

Low pH for both adults and larvae

E4

1356

764 (659)

  1. Unique matches against Swissprot are in brackets. Treatments: In the experimental manipulations the “C” signifies ambient or control conditions of ~400 µatm; the “E” signifies low pH (~1000 µatm, denoted “E”). CE: Adults cultured under ambient conditions with larvae spawned into low pH (samples CE2-CE4); E: larvae spawned into low pH produced from adults also cultured at low pH (samples E2-E4). For the molecular analyses, the transcriptional profiles of all treated larvae were compared against a set of age-matched larvae spawned into ambient conditions produced from ambient cultured adults.