Figure 6

Morphological changes of liver of L. crocea under low temperature and during subsequent rewarming with HE staining. (A,D) Liver of individuals in the control group (×400 and ×1000, respectively). The hepatocyte density in the liver were relatively high and the nucleus was located on the side of the cell, with less vacuolization. (B,E) Liver of individuals in the low temperature group (×400 and ×1000, respectively). The density of hepatocytes decreased obviously, as well as cell swelling and vacuolization increasing, with cell membrane disruption of the local liver. (C,F) Liver of of individuals in the rewarming group (×400 and ×1000, respectively). The density of hepatocytes increased and vacuolization decreased, compared with the low temperature group. Figure note: Hepatocyte (H), vacuolation (V), cell disruption (CD).