Table 1 Developmental process of megasporocytes after pollination in P. simonii × P. nigra var. italic.

From: An improved method for high-temperature induced embryo sac chromosome doubling in Populus simonii Carr. × P. nigra var. italica (Moench.) Kochne, an interspecific hybrid of Tacamahaca and Aigeiros poplars

Time after pollination (h)

Embryo sac percentage in each developmental stage (%)

*Percentage of inducible embryo sacs (%)

Meiosis I

Meiosis II

Uni-nucleate embryo sac

Two-nucleate embryo sac

Four-nucleate embryo sac

Eight-nucleate embryo sac

Mature embryo sac and post

0

12.70

28.57

36.51

14.29

7.94

  

58.73

12

5.00

33.33

35.00

18.33

6.67

1.67

 

60.00

24

2.94

32.35

35.29

19.12

8.82

1.47

 

63.24

36

3.17

19.05

36.51

25.40

11.11

4.76

 

73.02

48

 

15.15

30.30

31.82

13.64

6.06

3.03

75.76

60

  

26.87

34.33

22.39

10.45

5.97

83.58

72

  

16.42

32.84

17.91

19.40

13.43

67.16

96

  

5.08

20.34

22.03

28.81

23.73

47.46

120

    

18.03

44.26

37.70

18.03

144

     

39.62

60.38

0.00