Table 3 High temperature induction of embryo sac after pollination in P. simonii × P. nigra var. italic.

From: An improved method for high-temperature induced embryo sac chromosome doubling in Populus simonii Carr. × P. nigra var. italica (Moench.) Kochne, an interspecific hybrid of Tacamahaca and Aigeiros poplars

 

Time after pollination (h)

Temperature (°C)

Treatment duration (h)

No. of seeds

No. of seedlings

No. of triploids

No. of tetraploids

1

60

38

4

96

77

0

0

2

60

41

3

22

17

0

0

3

60

44

2

5

0

0

0

4

66

38

3

132

104

0

0

5

66

41

2

53

47

11

1

6

66

44

4

0

0

0

0

7

72

38

2

155

143

0

0

8

72

41

4

0

0

0

0

9

72

44

3

4

0

0

0

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