Figure 6

Reduced hepatic gene expression for lipogenesis mediators in DNT5 mice. All data were from mice which had been provided with western-style diet and DOX for 12 weeks. All mice were ApoE^−/−. (A) Example liver sections stained with H&E. Scale bar 100 μm. (B) Comparison of lipid content (steatosis) between Controls and DNT5. On the left, Mann-Whitney analysis showing mean and s.e.m (N = 11 Control, N = 14 DNT5). On the right, linear random effects model showing the standardised mean difference and confidence intervals. Data below the zero line indicate decrease in the DNT5 group (n/N = 8/11 Control, n/N = 8/13 DNT5). (C) All data were from quantitative real-time PCR analysis of liver RNA. Comparison of expression of sterol regulatory element-binding protein 1c gene (Srebp1c), acetyl-CoA carboxylase 1 gene (Acaca), fatty acid synthase gene (Fasn) and stearoyl-CoA desaturase 1 gene (Scd1) mRNA abundance between Controls and DNT5. On the left, Mann-Whitney analysis showing mean and s.e.m (N = 11 Control, N = 14 DNT5). On the right linear random effects model showing the standardised mean difference and confidence intervals. Data below the zero line indicate decrease in the DNT5 group (n/N = 8/11 Control, n/N = 8/13 DNT5). (D) Comparison of non-fasting plasma concentrations of cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides between Controls and DNT5 after 12 weeks of western-style diet and DOX administration. On the left, Mann-Whitney analysis showing mean and s.e.m (N = 11 Control, N = 14 DNT5). On the right, linear random effects model showing the standardised mean difference and confidence intervals (n/N = 8/11 Control, n/N = 8/14 DNT5).