Figure 2
From: In vivo assessment of increased oxidation of branched-chain amino acids in glioblastoma
(a) Metabolic pathway of hyperpolarized (HP) [1-13C]KIC. 13C-labeled KIC and its metabolic products are highlighted in red. (b) An in vitro time-averaged spectrum from F98 cells showed the injected HP [1-13C]KIC and produced [1-13C]leucine and H13CO3− peaks. (c) The corresponding time courses of HP KIC and the products. (d–e) In vivo chemical shift imaging of a F98 glioma-bearing rat using HP [1-13C]KIC and (f) the contrast-enhanced (CE) T1-weighted 1H image. Metabolite distributions of [1-13C]leucine and H13CO3− in a tumor-bearing rat brain slice after an injection of HP [1-13C]KIC. (g–h) The reconstructed spectra in the glioma (solid red) and the contralateral normal-appearing brain (NAB; dotted blue) and (i) in vivo metabolite ratio of [1-13C]leucine to H13CO3−.
