Table 1 Tumor burden calculation performed for different samples using three different methods: (1) from the tumor area segmented manually in 2D images of H&E-stained specimens; (2) from the tumor area segmented using a semi-automated method and micro-CT slices of I2E-stained specimen; and (3) and tumor volume reported as the sum of measurements of all the tumorous areas of all virtual micro-CT slices.

From: A step towards valid detection and quantification of lung cancer volume in experimental mice with contrast agent-based X-ray microtomography

Sample

Tumor burden (%)

H&E histological slice

micro-CT virtual slice

micro-CT volumetric image

1a

6.9

7.6

16.9

2b

5.9

—

20.7

3c

20.5

22.8

65.8

4d

3.3

10.5

8.8

  1. Columns from left to right: aFigs 3D and 4A,D and ; bFigs 3E and 4B,E; cFigs 3F and 4C,F (respectively equal to Fig. 5A,C); dFig. 5B,D, and micro-CT volumetric image not shown.