Figure 4

Detergent screening. (A) Percentage of fluorescence of solubilized membranes compared to lysate total fluorescence after solubilization with different detergents. Samples were read in a plate reader with a combination of excitation and emission filters of 485/520 nm, respectively. UDM (n-Undecyl-β-D-Maltopyranoside), SB3-12 (Sulfobetaine 3-12), OG (Octyl-β-Glucoside), NDM (n-Nonyl-β-D-Maltopyranoside), MEGA 8, 9, 10 (N-Octanoyl, N-nonanoyl, N-decadyol)-(N-Methylglucamine), LDAO (N,N-Dimethyldodecylamine N-Oxide), LAPAO (3-Dodecylamido-N, N′-Dimethylpropyl Amine Oxide), DMNG (Decyl Maltose Neopentyl Glycol), DDAO (Decyldimethylamine-N-Oxide), DM (Decyl-β-D-Maltopyranoside), DDM (Dodecyl-β-D-Maltoside), Cymal 7 (7-Cyclohexyl-1-Heptyl-β-D-Maltoside), Cymal 6 (6-Cyclohexyl-1-Hexyl-β-D-Maltoside), Cymal 5 (5-Cyclohexyl-1-Pentyl-β-D-Maltoside), C12E5 (Dodecyl Pentaethylene Glycol Ether), C12E8 (Octaethylene Glycol Monododecyl Ether), C12E9 (Dodecyl Nonaethylene Glycol Ether), C8E5 (Tetraethylene Glycol Monooctyl Ether), C8E5 (Octyl Pentaethylene Glycol Ether), CHAPS (3-[(3-Cholamidopropyl) Dimethylammonio]-1-Propanesulfonate), CHAPSO (3-[(3-Cholamidopropyl) Dimethylammonio]-2-Hydroxy-1-Propanesulfonate), F10 (n-Decylphosphocholine), F11 (n-Undecylphosphocholine), F12 (n-Dodecylphosphocholine). (B) FSEC chromatogram in a Superdex 200 HR column of membranes solubilized for 2 hours with selected detergents at 1% (w/v) concentration.