Figure 5 | Scientific Reports

Figure 5

From: Structural biology workflow for the expression and characterization of functional human sodium glucose transporter type 1 in Pichia pastoris

Figure 5

hSGLT1 purification. (A) SEC chromatography after Ni-NTA purification of WT-hSGLT1 with a Superdex 200 HR column of 25 mL and its corresponding SDS-PAGE on the right. Protein was run in TKCL at 0.2% (w/v) of Fos-12 at a flow of 0.4 mL/min. SEC collected fractions of 500 µL (2–5) and 1 mL (1 and 6) are represented on the SDS-PAGE. (B) SEC chromatography after Ni-NTA and FLAG-tag purification of WT-hSGLT1 with a Superdex 200 HR column of 25 mL and its corresponding SDS-PAGE on the right. Protein was run in TKCL at 0.2% (w/v) of Fos-12 at a flow of 0.4 mL/min. SEC collected fractions of 500 µL (1–6) were loaded and resolved in a SDS-PAGE.

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