Figure 2
Rest-activity parameters. (A) Daily activity pattern of each group (seconds/5 minutes) during the sleep restriction (SR) period. Each line represents the mean activity (±SEM) across the 21 days of SR. ZT0 = lights on and the beginning of gentle handling until ZT6 when recording started. n = 6 per group for CT (SHAM-CT and ROT-CT) and n = 12 per group for SR (SHAM-SR and ROT-SR). (B) Activity profile 15 minutes before and 2 hours after the onset of darkness (ZT12) during weeks 2 and 3 after the start of SR. Each line represents the mean activity (seconds/5 minutes) of the 14 days (±SEM). Ticks on x axis represent 5-minutes blocks. *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001 (two-way ANOVA followed by Fisher’s LSD test). Orange (*) = ROT-CT compared to ROT-SR; Blue (*) = ROT-SR compared to SHAM-SR; Green (*) = ROT-SR compared to SHAM-SR. n = 6 per group for CT (SHAM-CT and ROT-CT) and n = 12 per group for SR (SHAM-SR and ROT-SR). (C) Daily activity pattern each week of the 3 weeks of SR. Each line represents the mean activity (seconds/5 minutes) of 7 days ± SEM. ZT0 - lights on and the beginning of gentle handling until ZT6 when recording started. n = 6 per group for CT (SHAM-CT and ROT-CT) and n = 12 per group for SR (SHAM-SR and ROT-SR). (D) Total activity/day (h) in each of the 3 weeks of SR. The bars represent the mean ± SEM. *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001 (two-way ANOVA followed by Fisher’s LSD test). n = 24 SHAM-CT; n = 24 SHAM-SR; n = 20 ROT-CT; n = 20 ROT-SR.
