Figure 3
OPLS-DA plots in LC/MS targeted metabolomics data. (A) OPLS-DA scores plot showing separation by phenotype between the sham control group (SHAM-CT, dark blue, n = 11) and the rotenone control (ROT-CT, orange, n = 10); R2X 0.636; R2Y 0.996; Q²Y 0.312; P < 0.05. (B) OPLS-DA loading plot of SHAM-CT vs ROT-CT. Negative p(corr) values represent decreased and positive p(corr) values represent increased metabolite concentrations in the rotenone group (ROT-CT) compared to sham group (SHAM-CT). The metabolite bars are colour coded according to metabolite class as follows: amino acids and biogenic amines (blue); acylcarnitines (green); lysophosphatidylcholine acyl (lyso PC a) (dark orange); phosphatidylcholine diacyl (PC aa) (yellow); phosphatidylcholine acyl-alkyl (PC ae) (light orange); sphingolipids (SM) (brown). (C) OPLS-DA scores plot showing separation by sleep restriction alone (blue, SHAM-CT, n = 11; green, SHAM-SR, n = 12); R2X 0.421; R2Y 0.934; Q²Y 0.525; P = 0.04. (D) OPLS-DA loading plot of SHAM-CT vs SHAM-SR. Negative p(corr) values represent decreased and positive p(corr) values represent increased metabolite concentrations in sleep restriction (SR) compared to control. (E) OPLS-DA scores plot for SHAM-CT vs ROT-SR (dark blue, SHAM-CT, n = 11; dark red, ROT-SR, n = 11); R²X 0.499; R²Y 0.920; Q²Y 0.566; P = 0.03. (F) OPLS-DA loading plot of SHAM-CT vs ROT-SR. Negative p(corr) values represent decreased and positive p(corr) values represent increased metabolite concentrations in rotenone and SR compared to sham control.
