Figure 5
TAARs are important in mediating the effects of cadaverine. (A) Patient data was accessed at kmplot.com. Kaplan-Meier plots show the correlation between the mRNA expression of human TAARs and survival in breast cancer. (B) 4T1 cells were treated with, 100 nM cadaverine (48 hours) or 100 nM cadaverine (48 hours) in combination with 5 µM NF449 G protein inhibitor (last 48 hours of cadaverine treatment), cells were stained with Texas Red-X Phalloidin- and DAPI then cells were assessed by confocal microscopy (n = 2 in triplicates). (C) MDA-MB-231 cells were transfected with control si RNA or TAAR si RNA. The silencing efficacy was measured by Western blot (n = 2). (D,E) MDA-MB-231 cells were treated with vehicle or 0.3 μM cadaverine and transfected with control si RNA or TAAR si RNA. After 48 hours of incubation (D) TexasRed-X Phalloidin immunocytochemistry (p = 0.04 between cadaverine and TAAR1, p = 0.007 between cadaverine and TAAR8, p = 0.003 between cadaverine and TAAR9) (n = 1 in triplicates), and (E) MMP9 immunocytochemistry (p = 0.006 between control and TAAR1; p = 0.001 between cadaverine and TAAR8; p = 0.004 between cadaverine and TAAR9) (n = 1 in triplicates) were performed. CAD – cadaverine, other abbreviations are in the text.
