Figure 6
Cadaverine biosynthesis is suppressed in early stages of breast cancer. (A) Human fecal DNA samples were collected from 48 patients with different stages of breast cancer, and from 48 healthy patients. The abundance of DNA coding for CadA and LdcC of the indicated bacterial species were determined in the fecal DNA samples by RT-qPCR. Median values are indicated by a line. (B) Human fecal samples were collected from stage 1 breast cancer patients (n = 7) and from healthy (control) subjects (n = 3). The E. coli LdcC protein level was determined using Western blot. Hereby we show a representative image. Band intensity was normalized to total protein content assessed by ponceau-S staining of whole blots. Box chart of LDC protein expression from fecal samples (p = 0.003). Data was normalized to the mean of control samples. Blots were routinely cut as the representative blot. (C,D) Patient data was accessed at kmplot.com. Kaplan-Meier plots the correlation between the mRNA expression of human LDC and survival in breast cancer. Graphs show the correlation between LDC and disease survival in different forms and stages of the disease. Those arrays were also included where ER status was deducted from gene expression data. **Indicate statistically significant difference between control and treated groups at p < 0.01. Significance was calculated using two-sample student t-test (two-tailed). All abbreviations are in the text. Values on (A) were log2 transformed.
