Table 2 Stability ranking of candidate reference genes.

From: Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica

GeNorm

NormFinder

Bestkeeper

ΔCt Method

RefFinder

Gene name

Stability value

Gene name

Stability value

Gene name

Standard deviation

Gene name

Average SD

Gene name

Geomean of ranking values

HLN + Liver

HSP90AA1

0.608

PGK1

0.135

PGK1

0.630

PGK1

1.330

PGK1

1.320

GYPC

0.608

HSP90AA1

0.546

B2M

1.020

HSP90AA1

1.400

HSP90AA1

2.000

PGK1

0.741

SDHA

0.700

G6PD

1.760

B2M

1.440

GYPC

3.460

B2M

0.775

B2M

0.771

SDHA

0.710

GYPC

1.490

B2M

3.940

ACTB

0.812

GUSB

0.892

RPLP0

1.310

ACTB

1.530

SDHA

4.740

RPLP0

0.902

GYPC

0.935

GUSB

0.640

RPLP0

1.630

GUSB

5.180

G6PD

1.038

ACTB

0.941

ACTB

1.070

SDHA

1.640

ACTB

5.920

SDHA

1.168

RPLP0

1.190

HSP90AA1

0.710

GUSB

1.740

RPLP0

6.930

GUSB

1.261

G6PD

1.609

GYPC

1.060

G6PD

1.940

G6PD

8.450

TUBB

1.816

TUBB

3.970

TUBB

3.220

TUBB

4.040

TUBB

10.000

HLN

HSP90AA1

0.420

HSP90AA1

0.393

PGK1

0.450

HSP90AA1

0.760

HSP90AA1

1.000

GYPC

0.420

PGK1

0.423

B2M

0.440

PGK1

0.780

GYPC

2.060

PGK1

0.530

GYPC

0.440

G6PD

1.340

GYPC

0.790

PGK1

2.630

SDHA

0.550

B2M

0.481

SDHA

0.450

SDHA

0.820

B2M

4.160

B2M

0.586

SDHA

0.510

RPLP0

0.780

B2M

0.830

SDHA

4.470

GUSB

0.644

RPLP0

0.530

GUSB

0.500

RPLP0

0.870

GUSB

6.930

TUBB

0.692

ACTB

0.618

ACTB

0.810

ACTB

0.920

RPLP0

6.930

RPLP0

0.738

GUSB

0.731

HSP90AA1

0.270

GUSB

0.950

TUBB

7.940

ACTB

0.774

TUBB

0.779

GYPC

0.330

TUBB

0.990

ACTB

7.940

G6PD

0.923

G6PD

1.434

TUBB

0.640

G6PD

1.520

G6PD

10.000

Liver

GUSB

0.426

GYPC

0.294

PGK1

0.530

GYPC

0.920

GYPC

1.000

GYPC

0.426

PGK1

0.318

B2M

0.560

GUSB

0.940

GUSB

1.860

ACTB

0.533

GUSB

0.331

G6PD

0.950

PGK1

0.950

PGK1

3.500

HSP90AA1

0.564

HSP90AA1

0.516

SDHA

0.690

HSP90AA1

1.010

ACTB

3.870

PGK1

0.642

ACTB

0.554

RPLP0

0.780

ACTB

1.030

HSP90AA1

4.000

SDHA

0.684

SDHA

0.588

GUSB

0.330

SDHA

1.070

SDHA

6.240

B2M

0.758

B2M

0.666

ACTB

0.410

B2M

1.080

B2M

6.740

RPLP0

0.886

RPLP0

1.190

HSP90AA1

0.510

RPLP0

1.370

RPLP0

8.000

G6PD

0.984

G6PD

1.371

GYPC

0.330

G6PD

1.500

G6PD

9.000

TUBB

1.187

TUBB

1.926

TUBB

1.270

TUBB

2.000

TUBB

10.000

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