Table 2 Optimal cutoffs (1+ cytoplasmic inclusions for the mucin stains) and the sensitivity and specificity for different mucin stains and immunohistochemical markers to separate adenocarcinomas, large cell carcinomas and sarcomatoid carcinomas from squamous cell carcinomas and neuroendocrine tumors based on 657 primary lung cancers.

From: Mucin staining is of limited value in addition to basic immunohistochemical analyses in the diagnostics of non-small cell lung cancer

Staining

Cutoff

Sensitivity (%)

Specificity (%)

AUC

PASD

1+ inclusions

80

90

0.87

10+ inclusions

50

98

0.74

ABPAS

1+ inclusions

77

92

0.85

10+ inclusions

47

96

0.72

Mucicarmine

1+ inclusions

58

96

0.78

10+ inclusions

31

100

0.65

TTF-1 clone SPT24

≥10%

88

87

0.89

TTF-1 clone 8G7G3/1

≥1%

86

90

0.89

Napsin A

≥1%

86

98

0.93

CK7

≥50%

96

65

0.81

p40

<10%

100

81

0.91

p63

<50%

96

82

0.88

CK5

<1%

99

84

0.92

  1. Abbreviations: ABPAS, Alcian blue–periodic acid-Schiff; AUC, area under curve; CK, cytokeratin; PASD, Periodic acid-Schiff with diastase; TTF-1, thyroid transcription factor 1.
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