Figure 1
From: Simultaneous acoustic and photoacoustic microfluidic flow cytometry for label-free analysis
Conceptual schematic illustration of the acoustic flow cytometry system. (a) The illustration shows the overall design of a microfluidic device with collinearly aligned US transducer and laser focusing optical objective. (b) Hydrodynamic 3D flow focusing of a sample flow in the microfluidic device. The isometric view shows how the sample flow infused through the embedded needle at the cross-junction is 3D focused by the sheath flows from the side channels. The top and side views illustrate the position of the needle relative to the channel geometry. Gaps above, below, and on the sides of the needle allow the sheath flows to push the sample flow to the center of the channel. (c) The magnified view of a collinearly aligned transducer and laser beam. The incident US wave and laser beam interact with a particle at the interrogation zone producing both US backscatter and PA waves. (d) Image of an experimental setup that shows how the laser is focused on the interrogation zone of the US transducer.
