Figure 1 | Scientific Reports

Figure 1

From: Heterodera glycines utilizes promiscuous spliced leaders and demonstrates a unique preference for a species-specific spliced leader over C. elegans SL1

Figure 1

MH119144 is SL trans-spliced by a novel SL sequence. (A) Pairwise sequence alignment of C. elegans SL1 (CeSL1) and the first 22 nucleotides of MH119144 using EMBOSS needle with default settings. (|) = matching nucleotides, (.) = mismatched nucleotides, (−) = gap. (B) Secondary structural prediction of the putative MH119144 SL RNA consensus sequence using RNAfold shows a characteristic 3-hairpin SL RNA structure. (C) RT-PCR using H. glycines gDNA (g), cDNA (c) or no template DNA (NT). All lanes use a gene-specific reverse primer with either a gene-specific (GSP) forward primer or a SL forward primer.

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