Figure 4 | Scientific Reports

Figure 4

From: Doxorubicin-induced cardiomyopathy associated with inhibition of autophagic degradation process and defects in mitochondrial respiration

Figure 4

Expression of mitochondrial dynamics and OXPHOS regulatory protein in the hearts of acute Dox-cardiomyopathy mice. (A) Representative Western blot of the whole cell fraction showing expression of mitochondrial dynamic regulatory proteins in the acute Dox-treated hearts: Drp1, OPA1, and MFN2. Ponceau S protein staining of the transfer membrane confirmed approximately equal loading across the gel. (B) Representative Western blot showing expression of Complex I, Complex II, Complex III, Complex V and PDH complex protein derived from the whole cell fraction isolated from acute Dox-treated hearts at different time points. Ponceau S protein stain of the transfer membrane was used to confirm approximately equal loading. (C) Densitometric quantification of the temporal changes in Drp1, OPA1, and MFN2 protein expression in Dox-treated hearts. (D) Densitometric quantification of OXPHOS complex and PDH complex protein. Bars represent mean ± SEM. n = 4 mice per group at each time point. P values were determined by Tukey’s post-hoc test. NS = not significant.

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