Figure 5 | Scientific Reports

Figure 5

From: Doxorubicin-induced cardiomyopathy associated with inhibition of autophagic degradation process and defects in mitochondrial respiration

Figure 5

Suppression of mitochondrial respiration in the hearts of acute Dox-cardiomyopathy mice. (A) Mitochondrial oxygen consumption rate (OCR) profiles in isolated mitochondria from 3 days after acute Dox-treated hearts. Arrow indicates the sequential addition of oligomycin (1 µM), FCCP (4 µM), and rotenone (0.5 µM) plus antimycin A (0.5 µM). OCR profile is expressed as pMolesO2/min/µg of protein. Graph showing OCR under (B) baseline as well as with the addition of (C) oligomycin, (D) FCCP, and (E) rotenone plus antimycin A. Key parameters of mitochondrial function, including (F) ATP turnover, (G) maximal respiration, (H) state apparent and (I) coupling efficiency were significantly decreased in Dox mice. Bars represent mean ± SEM. n = 4–6 mice per group. P values were determined by Tukey’s post-hoc test.

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