Figure 1

Protein-coding transcriptome of DA neurons isolated from ventral mesencephalons of E14,5 embryos expressing GFP in Th⁺ cells. (a) FACS sorting of GFP⁺ and GFP⁻ cell populations for subsequent RNA-seq and ATAC-seq analyses. (b) Th expression (red) assessed by immunofluorescence on GFP⁺ and GFP⁻ cells cultured for 90 minutes after FACS. Nuclei were stained using Hoechst (blue). (c) Th mRNA relative expression of 3 independent GFP⁺ cell populations used for RNA-seq (triangle, diamond and square), compared to their matching control GFP⁻ cell populations. The bar represents the mean of the 3 enrichment values. mRNA expression was normalized relatively to Tbp mRNA expression. (d) Pathway analysis (Panther 2016) performed on the 1500 most expressed protein-coding genes obtained from 3 independent RNA-seq datasets, excluding mitochondrial genes. (e) mRNA expression in Log10(FPKM) of numerous cellular subtype marker genes. Each circle represents mRNA expression of a marker gene from 1 RNA-seq, and the bar represents the mean of the 3 values. Error bars show standard error of the mean. The color code is as follow: pale yellow, dopaminergic progenitors; yellow, differentiating and differentiated dopaminergic neurons; green, serotonergic neurons; blue, GABAergic neurons; purple, glutamatergic neurons; mustard, noradrenergic neurons; brown, radial glial like cells; dark brown, pericytes; brown-orange, endothelial cells.