Figure 2

Expression levels of synapsin I/II proteins in the striatum of RF-EMF-exposed mice. (A) Total proteins extracted from the striatum of mice were subjected to SDS-PAGE electrophoresis and immunoblotted with antibodies against synapsin I and synapsin II. The expression levels of synapsin I/II are significantly reduced by RF-EMF exposure. Cropped, representative gel images are shown. (B) The protein levels of synapsin I/II were normalized relative to α-tubulin. Each bar shows the mean ± SEM of three independent experiments. (C) Representative immunofluorescence images of synapsin I/II in striatum. Scale bar = 100 μm. (D) Relative optical density (ROD). The ratio of the ROD was calibrated as %, with the sham designated as 100%. Each bar represents the mean ± SEM. Statistical significance was evaluated using two-tailed unpaired Student’s t-test (**P < 0.01, ****P < 0.0001).