Figure 7
From: 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish
GSH, GSSG, and protein-thiol levels in control and kdsrI105R mutant. Quantification of GSH (A), GSSG (B), GSH to GSSG ratio (C), and protein-thiol (D) in control and kdsrI105R mutant. Three groups of control and mutant were used for analysis and each group contains 5 larvae. Relative mRNA expression of glutathione reductase (gsr) and glutathione synthetase (gss) in control and mutant (E). The working model illustrating that deleterious mutations in kdsrI105R induced transcriptional upregulation of sphk2, resulted in S1P accumulation. S1P accumulation elevated oxidative stress and ER stress mediated mitochondrial injury, hepatic injury and then steatohepatitis. GSH depletion might sensitize kdsrI105R mutant to oxidative stress (F). Error bars indicate standard deviation of the mean. *P ≤ 0.05, **P ≤ 0.005.
