Figure 1 | Scientific Reports

Figure 1

From: A Surface Plasmon Resonance-based assay to measure serum concentrations of therapeutic antibodies and anti-drug antibodies

Figure 1

General scheme of the SPR-based assay for simultaneous determination of IFX and ATI concentrations in serum. The SPR apparatus was the ProteOn XPR36 Protein Interaction Array system (Bio-Rad), which has six flow channels which can immobilise up to six ligands on parallel strips of the same sensor surface (e.g. TNFα, IFX, suitable controls including IgG or an “empty” channel). The flow channels can be rotated 90° so that up to six analyte solutions can be flowed in parallel on all the immobilised ligands, creating a multi-spot interaction array (each spot indicated by *): for example, injection of six concentrations of the analyte standards gives interaction affinities or the calibration curves; and the injection of six serum samples can simultaneously evaluate their SPR-binding signals on TNFα, IFX and appropriate controls. The terms “ligands” and “analytes” are used throughout the manuscript as indicating the immobilised and the flowing interactants, respectively.

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