Figure 2
Linearity between specific SPR signal and serum concentrations of IFX (A) or ATI (B). Serum from healthy volunteers was spiked with IFX (CT-P13) or ATI to the indicated concentrations then diluted 30-fold before injection into the SPR instrument. (a) Representative sensorgrams obtained injecting simultaneously the six dilutions of IFX-spiked sera over immobilised TNFα, after subtraction of the SPR signals from the parallel empty surfaces (see Fig. 1) for the experimental design and Fig. S1 for raw data). (b) Representative sensorgrams obtained injecting simultaneously the six dilutions of ATI-spiked sera over immobilised IFX, after subtraction of the SPR signals from the parallel surfaces coated with IgG. (c) Linearity between IFX concentration and specific SPR signal (at the end of the dissociation phase). Points show the mean ± SD for six consecutive injections, each as in panel a. The mean r2 of the six straight lines is 0.997 ± 0.001; the equation obtained from the linear regression is: y = 69.01(±1.37) x + 8.36(±5.16). (d) Linearity between ATI concentration and specific SPR signal (at the end of the dissociation phase). Points show the mean ± SD for six consecutive injections, each as in panel b. The mean r2 of the six straight lines is 0.998 ± 0.002; the equation obtained from the linear regression is: y = 4.65(±0.07) x + 7.30(±1.34).
