Figure 5
From: A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae
Chromatin is likely unchanged in strains lacking terminal 15 kb of 7L. (a) Genetic elements overexpressed upon methyl methanesulfonate (MMS) treatment, hydroxyurea (HU) treatment, or in tlc1-Δ are indicated with “+” signs, according to39,44. (b,c) mRNA levels of indicated coding sequences in indicated strains were assessed by qPCR, normalized by ACT1 mRNA and by corresponding levels in TLC1 7L-WT strains. Error bars correspond to SD. *p-value < 0.05 obtained with one-tailed Student T-test. n ≥ 3. (d) Spores of the indicated genotype were spotted in parallel for three serial passages on YPD or YPD complemented with 5 mM Nicotinamide (NAM) and analyzed as in Fig. 2b,c (e). Adjusted P-values were obtained by the Wilcoxon rank-sum test with a false discovery rate correction *p-value < 0.05 and **p-value < 0.01. n = 8 for each strain set. See Supplementary Table 3 for detailed p-values.
