Figure 2
From: Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks
Specificity and sensitivity tests of MAS-PCR assay. (A) Lines 1–3: Electrophoretic pattern of known samples of R. sp. I, Lines 4–6: R. sanguineus s.s., Lines 7–8: mixed DNA (R. sp. I + R. sanguineus s.s.), M: 100 bp DNA ladder. (B) Electrophoretic pattern of mixed DNA between R. sp. I and R. sanguineus s.s. diluted at different ratios. Starting DNA concentration 5 ng/µl. Lines 1–4: ratios 1:10, 1:50, 1:100, and 1:1.000. Lines 5–8: ratios (1:10), (1:50), (1:100), (1:1.000). M: 100 bp DNA ladder. The gels in (A) and (B) are different gels. The original photos of the gels are shown in the Supplementary information.
