Figure 3
From: Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks
Rhipicephalus sanguineus s.l. individuals showing paternal mtDNA using MAS-PCR. Lines 1–3: heteroplasmic parental R. sp. I female, F1 larva and F1 nymph from the experimental cross ♀ R. sp. I × ♂ R. sp. I. Lines 4–6: heteroplasmic F1 larvae and F1 adult female from the experimental cross ♀ R. sp. I × ♂ R. sanguineus s.s. Lines 7–9: heteroplasmic females from Putignano. Lines 10–12: F1 larvae from the experimental cross ♀ R. sp. I × ♂ R. sanguineus s.s. showing paternal mtDNA. Lines 13–14: positive control for R. sp. I and R. sanguineus s.s., respectively. Line 15: negative control. M: DNA ladder 100 bp. The original photo of the gel is shown in the Supplementary information.
