Figure 6

Combination of thiostrepton and 5-FU increases DNA damage in colon cancer cells. Combination of thiostrepton and 5-FU increases DNA damage in (A) HCT116 wt and (B) HCT116 5-FU res cell lines. Both cells were treated with 1 µg/ml of 5-FU, 1 µM of thiostrepton and combination for 24 h and stained with DAPI, tubulin and γH2AX antibodies. Combination of thiostrepton and 5-FU increases DNA damage in both cells. The combination of 5-FU and thiostrepton significantly increases γH2AX staining in HCT116 cells compared to control and either drugs alone ***p < 0.01. The combination of 5-FU and thiostrepton significantly increases γH2AX staining in HCT116 5-FU Res cells compared to control and either drugs alone ***p < 0.001. Images were visualized by microscopy. Images: original magnification X 40. Images were quantified using ImageJ software.